Table 3 Gene ontology by a function of the differently expressed proteins in the liver and the kidney extracted with electroporation mapped with GOrilla42.

From: Molecular harvesting with electroporation for tissue profiling

GO term

Description

P-value*

FDR q-value**

Enrichment (N, B, n, b)***

GO:0003824

catalytic activity

4.64E-50

9.28E-47

1.75 (1731, 672, 480, 326)

GO:0016491

oxidoreductase activity

1.23E-37

1.23E-34

2.78 (1731, 197, 398, 126)

GO:0048037

cofactor binding

2.47E-27

1.64E-24

2.56 (1731, 141, 484, 101)

GO:0050662

coenzyme binding

9.02E-21

4.5E-18

3.12 (1731, 93, 376, 63)

GO:0036094

small molecule binding

2.65E-14

1.06E-11

2.13 (1731, 336, 230, 95)

GO:0031406

carboxylic acid binding

5.16E-12

1.72E-9

3.58 (1731, 49, 316, 32)

GO:0016829

lyase activity

1.47E-11

4.21E-9

3.50 (1731, 46, 333, 31)

GO:0043177

organic acid binding

2.7E-11

6.74E-9

3.44 (1731, 51, 316, 32)

GO:0042802

identical protein binding

6.05E-11

1.34E-8

2.46 (1731, 299, 127, 54)

GO:1901265

nucleoside phosphate binding

2.98E-9

5.95E-7

1.43 (1731, 257, 743, 158)

GO:0000166

nucleotide binding

2.98E-9

5.41E-7

1.43 (1731, 257, 743, 158)

GO:0016616

oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

4.38E-9

7.3E-7

4.04 (1731, 43, 229, 23)

GO:0016836

hydro-lyase activity

5.24E-9

8.05E-7

6.79 (1731, 17, 195, 13)

GO:0051287

NAD binding

6.27E-9

8.94E-7

3.31 (1731, 29, 415, 23)

GO:0003735

structural constituent of ribosome

1.69E-8

2.25E-6

2.67 (1731, 35, 518, 28)

  1. *‘P-value’ is the enrichment p-value computed according to the mHG or HG model. This p-value is not corrected for multiple testing of 731 GO terms. **‘FDR q-value’ is the correction of the above p-value for multiple testing using the Benjamini and Hochberg (1995) method. Namely, for the ith term (ranked according to p-value) the FDR q-value is (p-value * a number of GO terms)/i. ***Enrichment (N, B, n, b) is defined as follows: N - is the total number of genes. B - is the total number of genes associated with a specific GO term. n - is the number of genes in the top of the user’s input list or in the target set when appropriate. b - is the number of genes in the intersection. Enrichment = (b/n)/(B/N).
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