Figure 1
From: Proteasome activator PA200 regulates myofibroblast differentiation
PA200 is upregulated in IPF lungs. (a) Homogenates of human IPF and donor lung tissues were analyzed for PA200 and α-smooth muscle actin (αSMA) expression by Western blotting. Diagram shows densitometric analysis of PA200 expression relative to β-Actin levels and normalized to the mean signal obtained for donor tissues (Mann-Whitney U test, donor tissues n = 9, IPF tissues n = 13). (b) Representative immunohistochemistry staining of PA200, αSMA and cytokeratin-5 (KRT5) in serial lung sections from donors (n = 6) and IPF patients (n = 10). Arrows indicate PA200 overexpression in αSMA expressing myofibroblasts of fibroblast foci (FF) in IPF lungs. Dashed arrows indicate PA200 overexpression in KRT5 expressing bronchiolar basal cells in IPF lungs. Full-length immunoblots are presented in Supplementary Fig. S5.
