Figure 6

PA200 deficiency augments TGF-β1-induced myofibroblast activation. (a) Western blot analysis of PA200 and αSMA of phLF after silencing of PA200 for 24 h and subsequent treatment with TGF-β1 for 48 h. Figure shows representative Western blots of experiments performed with phLF from n = 4 donors. (b) Analysis of cell count of phLF treated as in (a) normalized to control of phLF from the same donor (Bonferroni’s multiple comparison test, phLF from n = 3 different organ donors). (c) Analysis of cell count of phLF treated 24 h after overexpression of PA200 with TGF-β1 for another 24 h. Cell numbers were normalized to the control of the same donor (Bonferroni’s multiple comparison test, phLF from n = 3 different organ donors). Full-length immunoblots are presented in Supplementary Fig. S5.