Figure 1

Screening for low and high temperature acclimation. Results of temperature screen. Shown in (A) are average growth rates (n = 3) for the 133 S. marinoi mutants included in the screen, displayed as percent of wild type growth rate at the respective temperature. Dashed lines indicate the 75% cutoff value considered a true phenotype at each respective temperature. Colored rectangles (8 °C, blue and 24 °C, red) mark the harsher selection criteria of 95% of wild type at 16 °C, applied to reduce the number of mutants for mapping. The dots representing the mutants passing the harsher selection criteria have been enlarged and labelled with their SMMC classification, with a black triangle representing the mutant exhibiting a compromised phenotype at both temperatures. In (B) a Venn diagram is shown of the distribution of phenotypes at the <75% level at 8 or 24 °C but with a 95% level for 16 °C.