Figure 1 | Scientific Reports

Figure 1

From: Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6G+ myeloid cells identified by exploiting histidine decarboxylase BAC-GFP transgenic mice

Figure 1

Structural configuration of the Hdc-GFP transgenic allele. (A) Structure of the parental 293-kb BAC clone containing all the exons and approximately 120-kb of 5′ and 148-kb of 3′ flanking sequence of the mouse Hdc locus (RP23-40N15), the targeting DNA fragment for insertion of the GFP cassette and the Hdc-GFP transgene after deletion of the neomycin (Neo) resistance cassette are depicted. The target DNA fragment contains 1.1-kb of the upstream promoter and 1.1-kb of the 1st intron sequences for the 5′ and 3′ homologous regions, respectively. The Neo cassette is flanked by FLP recombinase target (FRT) sequences. The horizontal black arrows indicate the length of the DNA. The gray arrows depict the PCR amplicons for genotyping and copy number analyses. (B) Results of the genotyping PCR with GFP- and CAT-primer pairs in wild-type (WT) and Hdc-GFP mice (line#1, L1; line#2, L2). CAT, chloramphenicol acetyltransferase, was used to detect the BAC vector sequences. (C) Relative copy number analysis of Hdc-GFP line#1 at six points in the Hdc locus. 1E in the Hdc locus and intron1 in the Beta-actin locus (Actb int1) are used as control loci for nontransgene insertion. Values are provided as the means ± SEM (standard error of the mean) in the bar graphs.

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