Figure 2 | Scientific Reports

Figure 2

From: Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6G+ myeloid cells identified by exploiting histidine decarboxylase BAC-GFP transgenic mice

Figure 2

GFP expression pattern in the various tissues of the Hdc-GFP transgenic mice. (A) GFP fluorescence detected by in vivo imaging system (IVIS). Line#1 Hdc-GFP mice show a robust GFP signal in the peritoneal cavity cells (PECs), brain (Br) and stomach (St). Note the strong GFP signal in the corpus of the stomach, while the pyloric region rarely shows a GFP signal. Line#2 mice show GFP fluorescence in the brain and stomach at a lower level than line#1 mice. WT, wild type, serves as a negative control. Hr; heart, Lu; lung, Thy; thymus, Li; liver, Int; intestine, Kd; kidney, Sp; spleen. (B) Quantitative analysis of the GFP fluorescence level of WT, line#1 and line#2 mice. The values are calculated by subtracting the WT background level from the GFP fluorescence levels of the Hdc-GFP mice. (C,D) Quantitative RT-PCR analysis of endogenous Hdc and transgene-driven GFP mRNA expression. Note that the GFP-positive corpus shows a much higher level of endogenous Hdc mRNA expression than the GFP-negative pyloric region of the stomach. (E,F) GFP immunoreactivity in the gastric mucosa (E) and hypothalamus (F) of the Hdc-GFP mouse. GFP immunoreactivity in the tuberomammillary nucleus (TMN) is indicated by a black arrowhead. All values are provided as the means ± SEM in the bar graphs.

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