Figure 4 | Scientific Reports

Figure 4

From: Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6G+ myeloid cells identified by exploiting histidine decarboxylase BAC-GFP transgenic mice

Figure 4

GFP expression in the bone marrow and peritoneal hematopoietic cells of the Hdc-GFP transgenic mice. (A,B) Representative GFP histogram of the bone marrow and peritoneal cavity cell fractions. GFP-positive (%) cells in each fraction are gated by the black lines. (C,D) GFP-positive proportion in the isolated hematopoietic cells from the bone marrow and peritoneal cavity of the line#1 (n = 7) and line#2 (n = 5) Hdc-GFP mice. Hematopoietic cells were separated into the following seven distinct fractions: FcεR−c-kit+; progenitor cells, FcεR+c-kit+; mast cells, FcεR+c-kit−; basophils, Gr1+CD11b−; granulocytes, Gr1+CD11b+; immature myeloid cells (IMCs), Gr1−CD11b+; macrophages, c-kit−Gr1−CD11b−; others (hematopoietic cells other than those above). See Supplementary Fig. S2 for the separation strategy. Data are presented as the means ± SEM in the bar graphs.

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