Figure 7
Genetic variants in differentially active enhancer alleles segregate in wild Anopheles coluzzii. Genetic variation observed in colonized and wild A. coluzzii was compared for the six studied enhancers. Representative short sequence alignments are shown (full-length alignments with additional samples in Supplementary File S2). Asterisks above sequence alignments indicate variant positions shared between the cloned A. coluzzii colony haplotypes used in luciferase assays and the natural population. The top sequence row in each alignment is the PEST genome reference sequence, followed by sequences of alleles tested by luciferase assays (boxed by rectangles), followed by sequences of wild A. coluzzii. Ambiguous nucleic acid codes are used for heterozygous sites only in wild samples because the cloned sequences from A. coluzzii colonies are haplotypes, which are unambiguous.
