Figure 4
Impact of PABPC1 silencing on RSV multiplication. A549 cells were treated by siRNA PABPC1 or non-targeting siRNA (control) for 48 h and then infected with RSV-Cherry at MOI 0.05. (a) Cell lysates were collected at 0, 24 and 48 h p.i, subjected to SDS-PAGE and probed by antibodies directed against PABPC1, N or M. The visualization of all proteins was realized by Stain Free revelation. Full blots are available in Supplementary Fig. S4. (b) Cherry fluorescence was measured at 24 and 48 h post-infection and is expressed as a percentage of the values found for the control siRNA. The data was collected on 5 experiments with each point performed in triplicate. The significance was tested with a two-tailed paired t-test (alpha = 0.05) using GraphPad Prism software (**p < 0.01 (0.0073); ***p < 0.001 (0.0000025)). The normality of the data was tested with a Shapiro-Wilk normality test (alpha = 0.05) using GraphPad Prism software (p = 0.4510 & p = 0.5696). (c) Cell lysates were collected at 48 h p.i and subjected to a plaque titration assay. Results are expressed as the number of plaques in the 10−5 dilution wells, in percentage of the total found for the control siRNA. The data was collected on 3 experiments and each point was performed in duplicate. The significance was tested with a paired t-test using GraphPad Prism software (**p < 0.01 (0.0081)). The normality of the data was tested with a Shapiro-Wilk normality test (alpha = 0.05) using GraphPad Prism software (p = 0.3417).
