Figure 2 | Scientific Reports

Figure 2

From: Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers

Figure 2

Schematic diagram of the CRISPR/Cas9 construct and the target sites of three anthocyanin modification genes. (a) Schematic diagram of the T-DNA region of the CRISPR/Cas9 binary vector carrying pcoCas9, which is a codon-optimized Cas9 for plants. Two sgRNA expression cassettes are tandemly aligned. (b) Schematic diagrams of the Gt5GT, Gt3′GT, and Gt5/3′AT genes and their sequences, including the sequences of the amplified regions. Shaded boxes represent targets 1 and 2, whereas PAM refers to the protospacer adjacent motif (NGG). The primers used for the PCR amplification or direct sequencing are presented.

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