Table 1 NGS analysis of PCR amplicons of DNA and RNA of genome-edited lines.
Target gene | Line | Allele | No. of counts (DNA) | No. of counts (RNA) | Target 1 | Target 2 | ||
|---|---|---|---|---|---|---|---|---|
Sequence | In/Del | Sequence | In/Del | |||||
Gt5GT | WT | 1 | 12,79–8 | 50,836 | GTTAGAGGAAGAGGCCTTGC AGG | 0 | GCAAAAGGGCTCCTCGCGAG TGG | 0 |
2 | 11,604 | 26,024 | GTTAGAGGAAGAGGCCTTGC AGG | 0 | GCAAAAGGGCTCCTCGCGAG TGG | 0 | ||
#47 | 1 | 9,611 | 6,443 | GTTAGAGGAAGAGGCCTTGC AGG | 0 | GCAAAAGGGCT-----CGAG TGG | −5 | |
2 | 9,605 | 36,768 | GTTAGAGGAAGAGGCCTTGC AGG | 0 | GCAAAAGGG---------AG TGG | −9 | ||
#106 | 1 | 18,816 | 29,042 | CGTTAGAGGAAGAGGCC-TGC AGG | −1 | GCAAAAGGG-------CGAG TGG | −7 | |
2 | 30,010 | 261,382 | CGTTAGAGGAAGAGGC----------------||----------------GAG TGG | −256 | ||||
Gt3′GT | WT | 1 | 5,666 | 4,852 | CCT GGGATTCGACATATCTGTTC | 0 | AACGACGCTGCAGCTAAATT TGG | 0 |
2 | 5,419 | 2,055 | CCT GGGATTCGACATATCTGTTC | 0 | AACGACGCTGCAGCTAAATT TGG | 0 | ||
3 | 5,825 | 2,018 | CCT GGGATTCGACATATCTGTTC | 0 | AACGACGCTGCAGCTAAATT TGG | 0 | ||
4 | 4,852 | 22,326 | CCT GGGATTCGACATATCTGTTC | 0 | AACGACGCTGCAGCTAAATT TGG | 0 | ||
#11* | 1 | 20,194 | 35,543 | CCT GGG------CATATCTGTTC | −6 | AACGACGCTGCAGCT-AATT TGG | −1 | |
2 | 34,884 | 461,515 | CCT GGG---------------------||--------------------AATT TGG | −221 | ||||
3 | ||||||||
4 | ||||||||
#35 | 1 | 7,186 | 3,004 | CCT -------GACATATCTGTTC | −7 | AACGACGCTGCAGCTAAATT TGG | 0 | |
2 | 7,223 | 33,855 | --||--ATTCGACATATCTGTTC | −10 | AACGACGCTGCAGCTAAATT TGG | 0 | ||
3 | — | — | not amplified | — | not amplified | — | ||
4 | — | — | not amplified | — | not amplified | — | ||
Gt5/3′AT | WT | 1 | 15,403 | 12,453 | CCT CTTTGTCTCTCACTCTAAAA | 0 | CCA TGCAAGACTATAAAGTGATC | 0 |
2 | 18,258 | 20,721 | CCT CTTTGTCTCTCACTCTAAAA | 0 | CCA TGCAAGACTATAAAGTGATC | 0 | ||
#50 | 1 | 16,376 | 15,213 | CCT CTTTGTCTCTCACTCTAAAA | 0 | ------||-------AAGTGATC | −30 | |
2 | 19,913 | 11,267 | CCT CTT-----||------ [202 base reversed] AGACTATAAAGTGATC | −27 | ||||
#60 | 1 | 7,872 | 1,272 | CCT CTTTGTCTCTCACTCTAAAA | 0 | CCA TGC----CTATAAAGTGATC | −4 | |
2 | 6,874 | 22,102 | CCT C---GTCTCTCACTCTAAAA | −3 | CCA TGC–ACTATAAAGTGATC | −3 | ||
