Table 2 NHP Zygote Cleavage Patterns in Relation to Pronuclear Cortical Orientation and the Zygotic A → V Axis Analyzed from TLVM Recordings.

From: Fertilization and Cleavage Axes Differ In Primates Conceived By Conventional (IVF) Versus Intracytoplasmic Sperm Injection (ICSI)

Insem type

N scored

Sperm MIP and pronuclear orientation to overlying cortex at NEBD

Cleavage initiation at last position of: (%)

Cleavage furrow angle relative to the animal pole [2nd PB] (%)

Cleavage type (%)

2nd PB between daughter blastomeres post division (%)

Blastomere size post division (%)

   

♂PN

♀PN

Othera

0–30°

31–60°

>60°

Mer

Eq

 

Equal

Not Equal

ICSI

19

cortical radial

15/19 (79)

1/19 (5)

3/19 (16)

13/19 (68)

4/19 (21)

2/19 (11)

13/19 (68)

6/19 (32)

16/19 (84)

7/19 (37)

12/19 (63)

ICSI

37

cortical para-tangential

25/37 (68)

0

12/37 (32)

26/37 (70)

5/37 (14)

6/37 (16)

26/37 (70)

11/37 (30)

31/37 (84)

15/37 (41)

22/37 (59)

Partho

5

NA

NA

0

5/5 (100)

3/5 (60)

2/5 (40)

0

3/5 (60)

2/5 (40)

5/5 (100)

4/5 (80)

1/5 (20)

  1. Insem: insemination; Mer: meridional cleavage; Eq: equatorial cleavage; NEBD: pronuclear envelop breakdown; Partho: parthenogenetic chemical activation by ionomycin/6-DMAP exposure; no sperm DNA contribution; NA: not applicable.
  2. aincludes random cortical and/or 2nd PB sites.