Figure 5
Quantitative proteomic analyses by high resolution nano LC-MS/MS. (A) Schematic overview of the experimental workflow used for the quantitative proteomic analyses by high resolution nano LC-MS/MS. A high number of peptide groups (i.e., 31012 and 29547 for DStr and NAc tissues, respectively) was used for protein identification and, out of these, more than 80% were used as unique peptides for protein quantification, attesting the high efficiency of peptide labeling (data not shown). (B,C) Venn diagrams showing the overlapping of proteins differentially expressed in NAc (B) and DStr (C) tissues under different conditions. (D) Compared conditions together with numbers of differentially expressed proteins. (E) Heatmap of differentially expressed proteins in DStr tissue samples of WT and KO mice. Down-regulated and up-regulated proteins are colored in green and red respectively. Colour gradients represent the strength of differential expression.
