Figure 1
Maximum clade credibility trees generated with the BEAST2 analysis the combined sequences (COI, 16 S, and H3 genes), and the shell morphologies and excrements of the extant representative of the Cyclophoroidea. The outgroups (Conus and Pomacea) are not shown. For convenience, we assign numbers to the major nodes (Table S2). Numbers on branches indicate Bayesian posterior probabilities. The node bars indicate a 95% CI for the divergence times. The principal nodes are named by nominal numbers. The green circles indicate the calibration points. (a) method A and (b) method B were calculated using fossil calibration. The photographs of shells in (a) represent each family [Megalomastomatidae: (1) Cochlostoma achaicum coeruleum, (2) Hainesia litturatum; Cyclophoridae: (3) Cyclophorus oshimanus, (4) Cyclotus campanulatus, (5) Lagocheilus sp., (6) Chamalycaeus laevis, (7) Cyathopoma africanum, (8) Nakadaella micron; Diplommatinidae: (9) Diplommatina immersidens, (10) Diplommatina electa; Pupinidae: (11) Pupinella rufa, (12) Schistoloma sectilabrum, (13) Pseudopomatias maasseni; Neocyclotidae: (14) Aperostoma burringtoni]. The scale bars indicate each shell size [(3) to (5): 1 cm, (6) to (8): 2 mm, (12) and (13): 1 cm]. For convenience, the family Alycaeidae is treated as Cyclophoridae based on Webster et al.22.
