Figure 3
Mapping of cellular proteins and areas sampled by SMA biopsy. GO analysis of data identifying subcellular locations of all proteins sampled from (a) CFs (n = 3) and (b) VSMCs (n = 3), with consistent probabilities for locations of protein sampling in each cell population. (Colour code for GO maps, showing confidence of protein sampling from these locations: p > 10−3 white; p 10−3–10−5 beige; p 10−5–10−7 pale orange; p 10−7–10−9 dark orange; p < 10−9 red). (c) Mapping of known and predicted protein-protein interactions in SMA-biopsies from CFs by STRING analysis. Likely subcellular localisation indicated as follows: cytoplasm (purple, 93 proteins); plasma membrane (blue, 57 proteins); actin binding (yellow, 33 proteins). Line thickness indicates confidence of protein-protein interaction, a confidence threshold of 0.7 (high confidence) was used for all indicated interactions. (d) Mapping of known and predicted protein-protein interactions in SMA-biopsies from VSMCs by STRING analysis. Likely subcellular localisation indicated as follows: cytoplasm (purple, 115 proteins); plasma membrane (blue, 43 proteins); actin binding (yellow, 25 proteins). Line thickness indicates confidence of protein-protein interaction, a confidence threshold of 0.7 (high confidence) was used for all indicated interactions. (e) Heat map of proteins in SMA-biopsies specific to CFs (red) or VSMCs (blue) cells, and proteins sampled from both cell types (both colours).
