Figure 1
From: Downregulation of GRK5 hampers the migration of breast cancer cells
Expression analysis of GRK5 and characterization of MDA-MB-231 TRIPZ-shGRK5. (A) Expression analysis of GRK5 in different breast cancer cell lines utilizing qPCR. The values are depicted as mean + SD in the left panel and as median + range in the right panel. Student’s t-test was used for statistical evaluation. (B) Expression analysis of GRK4 family members in MDA-MB-231 wild-type cells. (C) Characterization of MDA-MB-231 TRIPZ-shGRK5. GRK5 expression analysis and fluorescence microscopy pictures upon induction with 5 µg/ml doxycycline for 72 h. (D) Expression analysis of GRK4 family members at the indicated time points upon induction with 5 µg/ml doxycycline. N = 3, *p < 0.05, **p < 0.01, ***p < 0.001.
