Figure 1
From: Central metabolism of functionally heterogeneous mesenchymal stromal cells
Cord blood harbours two mesenchymal stem cell populations with distinct properties. LL- and SL-CBMSC showed divergent proliferation rates measured by MTT assay (A). Mean and standard deviation are represented; ns, not statistically significant; *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test, SL-CBMSC vs. LL-CBMSC at different time points). Differences were also noted for the morphology, as shown in the bright filed images (B) and for senescence, as shown in β-galactosidase-positive cells quantification (C). Median and interquantile range are represented; ***p < 0.001 (Student’s t-test, SL-CBMSC vs. LL-CBMSC). Senescence was confirmed by p16 and p21 gene expression (D). Mean and standard deviation are represented; *p < 0.05 (Student’s t-test, SL-CBMSC vs. LL-CBMSC). (E) Different osteogenic potential was determined by Alizarin Red S staining of calcium deposits generated upon differentiation stimuli. (F) The amount of Alizarin Red S incorporated was biochemically quantified. Mean and standard deviation are represented; *p < 0.05 (Mann-Whitney test, SL-CBMSC vs. LL-CBMSC). Scale bar for 1B and 1E is 200 µm.
