Figure 3

AZD1775 and IR enhanced apoptosis in HeLa and SiHa cells. Cells were treated with 100 nM AZD1775 1 h prior to IR (6 Gy). At 72 h after IR, light microscopy photographs were taken (×10) and annexin V was quantified by FACS analysis for each group (A). Apoptosis assay was performed by measuring active caspase-3 with ELISA. AZD1775 (100 nM) was added 1 h prior to IR (4 Gy) and active caspase-3 was measured 48 h after IR (B). All experiments were repeated three times. The error bar represents standard error of the mean (s.e.m.).