Figure 1
From: Development and validation of a targeted gene sequencing panel for application to disparate cancers
Performance metrics for PV1 and PV2. (A) Box plots presenting data comparisons between PV1 (n = 80 samples tested) and PV2 (n = 111 samples tested) for unique reads (in millions; M), percent duplication, target coverage and percent base coverage at >20x and >100x. (B) Mean coverage over coding regions of shared genes was compared between PV1 and PV2 after additional probes to target poorly-covered regions were added to the latter. (C) Changes to individual shared targets between PV1 and PV2 are also presented. (D) The final mean coverage for PV2 is presented following the addition of 139 new genes for a total of 451 targeted genes, as well as (E) the final percentage of targeted coding bases in PV2 with a percent mean coverage of >100x. Dashed lines in panels (B–E) represent mean coverage. ***p < 0.001, ns = not significant.
