Table 1 Gene-Specific Oligonucleotide Primers Used for qPCR.
From: Using high-throughput sequencing to explore the anti-inflammatory effects of α-mangostin
Gene | Serial number | Primer sequence | Size of the products (bp) |
|---|---|---|---|
β-actin | NM_031144.3 | Forward 5′-CCACCATGTACCCAGGCATT-3′ | 253 |
Reverse 5′-AGGGTGTAAAACGCAGCTCA-3′ | |||
Nbl1 | NM_031609.1 | Forward 5′-CAAGCCTGCGGCAAGGAACC-3′ | 144 |
Reverse 5′-TCCAGGAGGTTCTTCAGGTTCAGG-3′ | |||
Fads2 | NM_031344.2 | Forward 5′-TCTCAGATCACCGAGGACTTCAGG-3′ | 153 |
Reverse 5′-GCCATTGCCGAAGTACGAGAGG-3′ | |||
Cxcl17 | NM_001107491.1 | Forward 5′-GTAGGAGGCTCCAGGAAGATGGC-3′ | 193 |
Reverse 5′-TGGCAGGCTCTGGAGTGCTTG-3′ | |||
Ldlr | NM_001013938.1 | Forward 5′-GCTGGACCTGGACCGACCTC-3′ | 153 |
Reverse 5′-GCCTAGTATGCGAGATGACGATGC-3′ | |||
Atf6 | NM_001107196.1 | Forward 5′-GGCTTCCTCCAGTTGTTCTGTCTC-3′ | 120 |
Reverse 5′-GCTTCTCTTCCTTCAGTGGCTCTG-3′ | |||
Celsr2 | NM_001191110.1 | Forward 5′-CAGCCAGTGTCAGTGTAACCATCC-3′ | 84 |
Reverse 5′-CGTTGAGCCGCACCGTGTAC-3′ | |||
Ptgs2 | NM_017232.3 | Forward 5′-TTCCAGTATCAGAACCGCATTGCC-3′ | 143 |
Reverse 5′-CCGTGTTCAAGGAGGATGGAGTTG-3′ | |||
Ccl2 | NM_031530.1 | Forward 5′-GCAGGTCTCTGTCACGCTTCTG-3′ | 112 |
Reverse5′-GAATGAGTAGCAGCAGGTGAGTGG-3′ | |||
Il9r | NM_017021.1 | Forward 5′-CAAGGACCGTATCGTTGGAGTGAC-3′ | 81 |
Reverse 5′-AGTCTGGCCTCGTAGATGGTATCG-3′ | |||
Il23a | NM_130410.2 | Forward 5′-CCAGTGTGGTGATGGTTGTGATCC-3′ | 113 |
Reverse 5′-AGATGTCCGAGTCCAGCAGGTG-3′ | |||
Fras1 | NM_001191595.1 | Forward 5′-CACAAGGAGCCGAACTGACCAAG-3′ | 180 |
Reverse 5′-AACACCGCACTGATGACTCGTAAC-3′ | |||
Kcnh2 | NM_053949.1 | Forward 5′-GGTCATCTACACAGCCGTCTTCAC-3′ | 124 |
Reverse 5′-GAGGAGGTCCACTACAGCCAGAG-3′ | |||
Cxcl1 | NM_030845.1 | Forward 5′-CCGCTCGCTTCTCTGTGCAG-3′ | 155 |
Reverse 5′-GTCCTGGCGGCATCACCTTC-3′ | |||
Cd69 | NM_134327.1 | Forward 5′-CTTGTGCTGTGCTCGTAGTGGTC-3′ | 131 |
Reverse 5′-GCAGGAAGCAGCATGGTGGTC-3′ | |||
Sipa1 | NM_001004089.1 | Forward 5′-TGCTGCCTTACACGCCTAATAACC-3′ | 97 |
Reverse 5′-GCTCCTGGAACACGATGGTCAC-3′ | |||
IL-1β | NM_012762.2 | Forward 5′-GCAGGCAGTATCACTCATTGT-3′ | 221 |
Reverse 5′-GGCTTTTTTGTTGTTCATCTC-3′ | |||
IL-18 | NM_019165.1 | Forward 5′-TGATATCGACCGAACAGCCAACG-3′ | 88 |
Reverse 5′-GGTCACAGCCAGTCCTCTTACTTC-3′ | |||
caspase 1 | NM_012762.2 | Forward 5′-ATGGCCGACAAGGTCCTGAGG-3′ | 185 |
Reverse 5′-GTGACATGATCGCACAGGTCTCG-3′ | |||
Nlrp3 | NM_001191642.1 | Forward 5′-GAGCTGGACCTCAGTGACAATGC-3′ | 146 |
Reverse 5′-ACCAATGCGAGATCCTGACAACAC-3′ | |||
Trib3 | NM_144755.2 | Forward 5′-CCACATCTCTGGCTGCTTCTGC-3′ | 122 |
Reverse 5′- TGGTCCAGGCTCAGGCTCATC-3′ |
