Table 1 Primer sequences used for qPCR in this study.
From: Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells
Multi-species biofilm composition analysis | Reference | ||
|---|---|---|---|
Bacterial spp. | Forward primer 5′−3′ | Reverse primer 5′−3′ | |
Streptococcus spp. | GATACATAGCCGACCTGAG | TCCATTGCCGAAGATTCC | |
A. naeslundii | GGCTGCGATACCGTGAGG | TCTGCGATTACTAGCGACTCC | |
V. dispar | CCGTGATGGGATGGAAACTGC | CCTTCGCCACTGGTGTTCTTC | |
Fusobacterium spp. | GGATTTATTGGGCGTAAAGC | GGCATTCCTACAAATATCTACGAA | |
P. gingivalis | GCGCTCAACGTTCAGCC | CACGAATTCCGCCTGC | |
P. intermedia | CGGTCTGTTAAGCGTGTTGTG | CACCATGAATTCCGCATACG | |
A. actinomycetemcomitans | GAACCTTACCTACTCTTGACATCCGAA | TGCAGCACCTGTCTCAAAGC | |
Quantitative PCR gene expression analysis of epithelial and immune cells | |||
Cytokine | Forward primer 5′−3′ | Reverse primer 5′−3′ | |
IL1β | TCCCCAGCCCTTTTGTTGA | TTAGAACCAAATGTGGCCGTG | |
TNF | CCCCAGGGACCTCTCTCTAATC | GGTTTGCTACAACATGGGCTACA | |
IL6 | CAATCTGGATTCAATGAGGAGAC | CTCTGGCTTGTTCCTCACTACTC | |
IL8 | CAGAGACAGCAGAGCACACAA | TTAGCACTCCTTGGCAAAAC | |
IL10 | GCTGGAGGACTTTAAGGGTTACCT | CTTGATGTCTGGGTCTTGGTTCT | |
GAPDH | CAAGGCTGAGAACGGGAAG | GGTGGTGAAGACGCCAGT | |
