Figure 1 | Scientific Reports

Figure 1

From: Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone

Figure 1

Effect of CHC on RANKL-induced differentiation of BMMs into osteoclasts. (a–e) BMMs were cultured for 72 hours in the presence of M-CSF, RANKL, and various concentrations of CHC. (a) Osteoclasts were detected by TRAP activity staining. (b) TRAP activity in the cultures was determined. (c–e) The area of osteoclasts (c), total osteoclast area (d), and the number of osteoclasts (e) per well were quantified using the software package pre-installed in the optical microscope. (f) BMMs were cultured for 24, 48, and 72 hours in the presence of M-CSF and the indicated concentrations of CHC, which was followed by determination of cell number to evaluate BMM proliferation. (g) BMMs were cultured for 72 hours with M-CSF and various concentrations of RANKL in the presence or absence of CHC (0.3 mM). TRAP activity in the cultures was determined. (h) BMMs were cultured for 48 hours in the presence of M-CSF, RANKL, and various concentrations of CHC. Expression levels of Rank, Nfatc1, Dcstamp, Trap, Ctsk, and Irf8 are expressed as relative to that of Gapdh. (a) Scale bars, 500 μm. (b–h) Values are expressed as the mean ± SD. * and ** indicate significant difference at p < 0.05 and p < 0.01, respectively. n.s., not significant.

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