Figure 8
Expression of active Rap1a in RPE by adenovirus transduction does not increase autophagy and cell death. Western blots of (A,B) LC3A/B protein and (C,D) caspase 3 and cleaved caspase 3; and (J-K) TUNEL staining in human RPE transduced with adenovirus expressing GFP (Ad-GFP) or GFP and constitutively active Rap1a (Ad-63E) (A and C), (representative gel images and (B and D), quantification of densitometry *p < 0.05 vs. Ad-GFP; n = 3; CC in C refers to cytochrome C treated cell lysate.
