Figure 6
Comparison of the levels of DAB2IP mRNA expression in lung cell lines. (A) Basal expression levels of DAB2IP mRNA in 16HBE, and A549 cell lines (n = 3). Bars represent mean ± SD of 2^-ΔΔCT. DAB2IP mRNA expression. (B) 16HBE and (C) A549 stimulated with CSE w/wo GSK343for 14 days (n = 3). Bars show the levels of DAB2IP mRNA expressed as fold-change compared to untreated cells chosen as the reference sample. (D) Expression of H3K27me3 in A549 stimulated with CSE (10 and 20%) for 14 w/wo GSK343 8n = 3). Bars represent mean ± SD of arbitrary densitometric units (A.D.U.), normalized to β-actin used as the loading control. Representative Western blot analyses of H3K27me3 and β-actin was shown. Statistical analysis was performed by ANOVA test with Fisher’s correction for multiple comparisons. Significance was accepted at p < 0.05.
