Figure 9
Effect of CSE and EZH2 inhibitor GSK343 on Vimentin protein expression in A549 cells stimulated with chronic CSE for 14 days. (A) Vimentin analysis was performed by Western blot in A549 cell stimulated for 14 days with CSE 10–20% alone and in combination with GSK343(n = 3). Bars represent mean ± SD of arbitrary densitometric units (A.D.U.) normalized to β-actin. Representative Western blot analyses of Vimentin expression, and β-actin are shown. Statistical analysis was performed by ANOVA test with Fisher’s correction for multiple comparisons. Significance was accepted at p < 0.05. (B) Representative Vimentin expression in A549 cells stimulated with CSE (10–20%) w/wo GSK343 for 14 days (n = 3) by immunofluorescence analysis.
