Figure 1
From: β-catenin regulates the formation of multiple nephron segments in the mouse kidney
(A–E) Lineage analysis with Osr2Cre in the developing mouse kidney. Cre-mediated recombination activates expression of EYFP reporter, which labels Osr2-expressing cells and their descendants. (A) Osr2Cre targets the proximal (Wt1+) and medial (Jag1+) segments of the S-shaped body. In the nephron, Osr2Cre targets podocytes (B), proximal tubules (C), and loops of Henle (D), but not distal tubules (B). White arrowhead in (B) points to podocytes that escaped Osr2Cre. (E) From the absence of EYFP signal (green), we conclude that Osr2Cre targets neither the cap mesenchyme (white arrowhead, for example) nor the interstitial cells (asterisk). (F) β-catenin is ubiquitously expressed in the S-shaped body. (G) Osr2Cre removes β-catenin from the proximal (yellow arrowhead) and medial (white arrowhead) segments of the S-shaped body. Images are representative of three independent experiments. HT, Hoechst. Stage E18.5. Scale bar: 50 μm.
