Figure 3
From: β-catenin regulates the formation of multiple nephron segments in the mouse kidney
β-catenin is required for the proper formation of the renal corpuscle. (A) In the renal corpuscle of the control kidney, Wt1+ podocytes surround Pecam1+ endothelial cells in a crescent configuration and Akap12 marks parietal epithelial cells. By contrast, in the β-catenin mutant kidney, Pecam1 + endothelial cells fail to populate inside the renal corpuscle and Akap12 expression is considerably reduced. (B) Pecam1 + endothelial cells invade the vascular cleft in the S-shaped body in both control and mutant kidneys. In the proximal segment of the S-shaped body, Wt1 is detected in both visceral and parietal epithelial cells while Mafb is detected in the visceral epithelial cells only. The initial specification of visceral and parietal epithelial cells appears normal in the β-catenin mutant kidney. Images are representative of three independent experiments. HT, Hoechst. Stage E18.5. Scale bar: 50 μm.
