Figure 4
Neuronal volume is increased in Wfs1 KO animals. Neuronal cell volume in (a) medial nucleus; (b) dorsal nucleus; (c) principal nucleus; and (d) in all inferior olive (IO) nuclei together. There is an increased neuronal volume in saline-treated Wfs1 KO animals compared to WT littermates in all subnuclei, suggesting neuronal swelling in Wfs1 KO animals. (a) Liraglutide treatment decreased neuronal swelling in the medial nucleus in Wfs1 KO animals. (d) In Wfs1 KO animals there is an increased number of large neurons (1600 µm3 and above, dotted line), as compared to liraglutide-treated Wfs1 KO animals, indicating that liraglutide treatment prevented neurons from swelling. The data were compared using one-way ANOVA followed by Tukey’s HSD tests; *p < 0.05, **p < 0.01, ***p < 0.001. The data are presented as the mean ± SEM, n = 4–7 per group.
