Figure 8
Liraglutide treatment protected Wfs1 KO rats’ optic nerve axons from degeneration. Effect of 6 months of liraglutide treatment on Wfs1 KO rat optic nerve. Representative electron micrographs of (a) WT Sal and (b–d) KO Sal rats. Optic nerves of KO Sal rats display several features of axon degeneration resulting in (b,c) myelin collapse (arrowheads), (d) extensive vacuolization and electrondense axoplasm (arrowheads). (e) Liraglutide treatment protected KO animals from axonal area decline. Axon area per animal in percentage was compared. (f) Liraglutide treatment protected KO animals from axonal degeneration, measured as number of degenerated axons per 1000 axons. The data were compared using factorial ANOVA followed by Fisher’s LSD tests; *p < 0.05, **p < 0.01, ***p < 0.001. Scalebar: 5 μm. The data are presented as the mean ± SEM, n = 6 per group.
