Table 2 Output for the mixed-model nested ANOVA (fixed factors).

From: Effects of multiple stressors on river biofilms depend on the time scale

Response variable

Factor

df

SS

MS

F

P

Chlorophyll-a concentration

Hydrological stress (H)

1

2.81E + 02

2.81E + 02

57.6

<0.001

 

H:P

1

5.13E + 01

5.13E + 01

10.5

0.005

Photosynthetic efficiency

Hydrological stress (H)

1

6.76E + 05

6.76E + 05

249.4

<0.001

 

Pesticides (P)

1

1.37E + 05

1.37E + 05

50.7

<0.001

 

H:P

1

1.51E + 04

1.51E + 04

5.6

0.031

Photosynthetic capacity

Warming (W)

1

3.49E + 04

3.49E + 04

8.6

0.010

 

Hydrological stress (H)

1

2.48E + 05

2.48E + 05

61.1

<0.001

Basal chlorophyll fluorescence

Hydrological stress (H)

1

1.07E + 06

1.07E + 06

107.4

<0.001

 

W:P

1

5.42E + 04

5.42E + 04

5.5

0.033

 

W:H:P

1

5.09E + 04

5.09E + 04

5.1

0.038

Leucine aminopeptidase activity

Hydrological stress (H)

1

1.33E + 04

1.33E + 04

10.2

0.006

16S rRNA gene abundance

Warming (W)

1

1.56E + 15

1.56E + 15

33.7

<0.001

Substrate utilization richness

W:H

1

8.44E + 01

8.44E + 01

10.0

0.006

Gross primary production

Warming (W)

1

3.42E + 03

3.42E + 03

18.7

<0.001

 

Hydrological stress (H)

1

5.52E + 03

5.52E + 03

30.1

<0.001

 

Pesticides (P)

1

1.08E + 03

1.08E + 03

5.9

0.027

 

W:P

1

1.50E + 03

1.50E + 03

8.2

0.011

 

H:P

1

2.53E + 03

2.53E + 03

13.8

0.002

Community respiration

Warming (W)

1

4.15E + 03

4.15E + 03

41.3

<0.001

 

Hydrological stress (H)

1

1.65E + 03

1.65E + 03

16.5

<0.001

 

Pesticides (P)

1

1.63E + 03

1.63E + 03

16.2

<0.001

 

W:H

1

6.01E-01

6.01E-01

6.0

0.026

 

H:P

1

7.93E-01

7.93E-01

7.9

0.013

 

W:H:P

1

8.09E-01

8.09E-01

8.1

0.012

Production-respiration ratio

Hydrological stress (H)

1

5.71E + 01

5.71E + 01

35.7

<0.001

 

Pesticides (P)

1

3.69E + 01

3.69E + 01

23.1

<0.001

 

H:P

1

1.19E + 01

1.19E + 01

7.5

0.015

  1. Significant results for single and multiple stressors are presented (P-value < 0.05). Acronyms: H = hydrological stress, P = pesticides, W = warming, df = degrees of freedom, SS = sum of squares, MS = mean of squares, F = F-value, P = P-value. The strongest effect (i.e. highest F-value) for each response variable is highlighted in bold. Substrate utilization diversity does not appear in the table as none of the factors included in the ANOVA were significant (P > 0.05). For the complete ANOVA output, including non-significant effects and residuals, see supplementary information.
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