Figure 4
From: A high-speed, bright, red fluorescent voltage sensor to detect neural activity
Ace-mScarlet enables dual channel experiments using either green calcium indicators or blue-light-activated rhodopsins with minimal crosstalk. (a) Ace-7aa-mScarlet (top) and GCaMP6f (middle) facilitate parallel voltage and calcium recording in cultured neurons with minimal fluorescence crosstalk. Ace-mScarlet’s kinetics closely track individual action potentials (bottom) at 8 Hz. (b) Ace-mScarlet produces spikes within corrected fluorescent traces (top) that closely match action potentials elicited via optogenetic stimulation of CheRiff (bottom) with 8 Hz, 2 ms pulses of blue light.
