Figure 5 | Scientific Reports

Figure 5

From: A New Strategy for Identifying Mechanisms of Drug-drug Interaction Using Transcriptome Analysis: Compound Kushen Injection as a Proof of Principle

Figure 5

Differentially regulated genes shown in pathway and validation of selected gene regulation. (A) Comparison of types of regulation for CKI with doxorubicin and 5-Fu in the “Cell Cycle” pathway. Left half of the rectangle for each gene represents CKI with doxorubicin in A431 cells and the right half represents CKI with 5-Fu in MDA-MB-231 cells. Red and green colors mean synergistic and antagonistic regulation, respectively. (B) Validation of gene regulation at protein level. Four genes (HO-1, TNFAIP3, P21 and CBL) with opposite types of regulation in A431 and MDA-MB-231 cells identified by transcriptome sequencing were selected and validated by flow cytometry. ‘U’, ‘D’ and ‘C + D’ represent untreated, single chemotherapy drug treatment and CKI plus chemotherapy drug treatment, respectively. Data are represented as means ± SEM (N = 3). Statistical analyses were performed between single drug treated or combined treated to untreated with two-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001).

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