Figure 1

Maternal care assessment and developmental behavioural traits in WT, KO and KO ENRICHED mice. (A) Detailed representation of different maternal activities frequency in WT, KO and KO ENRICHED pups from PND 1 to PND 7. Data are presented as mean ± S.E.M. N = 6 WT, 6 KO and 5 KO ENRICHED. *p < 0.05 for KO and KO ENRICHED vs WT pups and §p < 0.05 for KO ENRICHED vs KO pups (Two-way ANOVA, followed by Tukey’s post-test analysis). (B) Eyes opening was evaluated in pups on PND 12. N = 37 WT, 37 KO and 21 KO ENRICHED. *p < 0.05 for KO and KO ENRICHED vs WT and §p < 0.05 for KO ENRICHED vs KO mice. (One-way ANOVA, followed by Tukey’s post-test analysis). (C) Social recognition was evaluated in the homing test on PND 14 by measuring the time in seconds (s) spent in the nest area, in the clean area and in the start area. Data are presented as mean ± S.E.M. N = 37 WT, 37 KO and 20 KO ENRICHED. *p < 0.05 for time spent in the clean area or start area vs time in nest area for WT; §p < 0.05 for time spent in the start area vs time in nest area for KO; #p < 0.05 for time spent in clean area vs time in nest area for KO ENRICHED and #p < 0.05 for time spent in start area vs time in nest area for KO ENRICHED (Two-way ANOVA, followed by Sidak’s post-test analysis). (D) Graphic representation of data collected during olfactory habituation/dishabituation test by measuring the time in seconds (s) spent by adolescent mice in sniffing cotton-tipped swabs saturated with different odours. Data are presented as mean ± S.E.M. N = 20 WT, 20 KO and 17 KO ENRICHED. (Two-way ANOVA, followed by Sidak’s post-test analysis).