Figure 2

Preventative and therapeutic effects of low-dose ionizing radiation on the degranulation and related signaling pathway. RBL-2H3 cells were irradiated before (preventative effect) and after (therapeutic effect) cells were activated with anti-DNP IgE and stimulated with DNP-HSA for 1 h. Following irradiation, we measured β-hexosaminidase (A) and histamine (B) in supernatants. (C) The phosphorylation of protein tyrosine kinases, p-Lyn, p-Syk, p-PKCα/β, δ, and p-PLCγ1, was detected by western blot analysis following stimulation with DNP-HSA for 10 min. Actin was used as a loading control. Each value represents means ± S.E. for three independent experiments and was analyzed by the t-test to determine statistical significance. * and # indicate a value of p < 0.05 for the difference between control vs the preventative and therapeutic group, respectively.