Figure 5
From: CRISPR interference to interrogate genes that control biofilm formation in Pseudomonas fluorescens
Biofilm phenotypes of genes involved in GacS and c-di-GMP signaling are consistent for CRISPRi silencing and deletion. (A) Surface rendering (IMARIS software) of reconstituted 3D confocal volume images of biofilm pellicles displayed in Fig. 4. Targeted genes encode the GacS (PFLU3777) kinase sensor and the c-di-GMP binding proteins RimA (PFLU0263, PDE), DipA (PFLU0458, PDE), Alg44 (PFLU0988) alginate co-polymerase, GcbA (PFL0621, DGC) and BifA (PFLU4858, PDE). The control corresponds to the pPFL-gRNA plasmid with no guide RNA inserted. (B) Comparison of changes in biofilm thickness caused by CRISPRi silencing and gene deletion.
