Figure 2 | Scientific Reports

Figure 2

From: Non-typeable Haemophilus influenzae isolates from patients with chronic obstructive pulmonary disease contain new phase-variable modA methyltransferase alleles controlling phasevarions

Figure 2

Silver stained protein gel showing outer-membrane protein (OMP) expression differences resulting from modA15 and modA18 phase-variation. OMPs were prepared using a sarkosyl OMP preparation protocol. 5 μg of each OMP fraction was separated on SDS PAGE and visualized using ammoniacal silver staining. Differences in protein expression between modA15 ON vs OFF, and modA18 ON vs OFF are highlighted with arrows. Differences were identified visually, and confirmed by processing each band with imageJ using a band that was observed as the same intensity in the respective ON vs OFF lane (loading control; LC). Only differences that were confirmed by ImageJ as >2-fold intensity difference ON vs OFF relative to the loading control are highlighted. Difference in intensity as fold difference ON vs OFF normalised to loading control. modA15 ON vs modA15 OFF: (i) 0.11 fold; (ii) 4.4 fold; (iii) 6.3 fold. modA18 ON vs modA18 OFF: (iv) 0.46 fold; (v) 0.40 fold. In addition, a difference in size of a protein of equivalent expression is highlighted with * in modA18 ON vs OFF. The full gel image is provided in Supplementary Fig. 1.

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