Figure 1

Multipotency characterisation in vitro. (a) Relative mRNA expression of MSC marker, CD90 and transcription factors regulating pluripotency, NANOG and Oct-4 after 1 week of non-induced culture after transport. Expression is presented relative to the Direct(AB + FGFlow) group (p < 0.05). (b) Alizarin stain showing cells from all culture conditions and from all donors differentiated consistently into the osteogenic lineage when induced. (c) Oil red O stain confirming lipid vacuoles when adipogenic lineage was induced.