Table 1 Description of the different isolation and expansion conditions for Donor I.

From: Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells

DONOR I

Abbreviation

Description

Direct(AB + FGFlow)

Bone marrow was directly seeded onto flasks with α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF2 (all from R&D)

ACK(AB + FGFlow)

Prepared with Ammonium-Chloride-Potassium (ACK) lysing buffer (Gibco) and cells were seeded with α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF-2

Lympho(AB + FGFlow)

Bone marrow was centrifuged in a density gradient medium Lymphoprep (Stemcell Technologies) to isolate mononuclear cells before culturing in α-MEM +10% AB serum +1% P/S and 5 ng/mL FGF2 (all from R&D)

Direct(PL)

Bone marrow was directly seeded onto flasks with α-MEM +1% P/S (Gibco) +10% PL + heparin (2I/U per mL)

  1. Abbreviations: α-MEM, alpha minimum essential medium; AB serum, human serum from type AB donors; P/S, penicillin/streptomycin, FGF2, fibroblast growth factor 2.
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