Figure 7
Structural mutant of the F1 domain due to amino acid substitution in homologous modelling. (a) To investigate the effectiveness of mutants in regard to their ability to affect the 2E4 epitope, clustered alanine substitution was used to test their contribution to the epitope. Mutation to alanine resulted in an obvious deformation of B2L involving the conformation of the F1 fragment. The hydrogen bond number between atoms was significantly increased in the new B2L model. (b) Deformation of B2L led to denaturation of the structure responsible for the binding activity to 2E4. Note: the red letters indicate replaced residues in the 2E4 epitope, while the yellow letters indicate nonvalent residues employed in this investigation.
