Table 1 Primers used to generate specific mutations in the B2L-F1 gene of the ORFV OV/HLJ04 strain for key amino acid investigation.
Mutants | The Synthesized oligonucleotides |
|---|---|
K61/A-F | 5-CGGATCCAGCTCCACCGCCGAGGG-3 |
K61/A-R | 5-CCTCGAGTTCCCGCGTGGACACCT-3 |
K69/A-F | 5-CGGATCCAGCTCCACCAAGGAGGGCGTCGACGTCAAAGACGCCCTCT-3 |
K69/A-R | 5-CCTCGAGTTCCCGCGTGGACACCTT-3 |
K75/A-F | 5-CGGATCCAGCTCCACCAAGGAGGGCGTCGACGTCAAAGACAAGCTCTGCACGCTCGCCGCCGAG-3 |
K75/A-R | 5-CCTCGAGTTCCCGCGTGGACACCTT-3 |
D94/A-F | 5-CGGATCCAGCTCCACCAAGGAGGG-3 |
D94/A-R | 5-CCTCGAGTTCCCGCGTGGACACCTTGACTTTGTAGTAGTTGATGCCCGCCGCGCGCAGCTCGGCCGC |
E95/A-F | 5-CGGATCCAGCTCCACCAAGGAGGG-3 |
E95/A-R | 5-CCTCGAGTTCCCGCGTGGACACCTTGACTTTGTAGTAGTTGATGCCCGCCGCGCGCAGGGCGTCCGC-3 |
L96/A-F | 5-CGGATCCAGCTCCACCAAGGAGGG-3 |
L96/A-R | 5-CCTCGAGTTCCCGCGTGGACACCTTGACTTTGTAGTAGTTGATGCCCGCCGCGCGGGCCTCGTCCGC-3 |
