Figure 4
From: A miRNA signature in endothelial cell-derived extracellular vesicles in tumor-bearing mice
EC-EVs from tumor-bearing mice are enriched in specific miRNAs. (a) Nanostring miRNA array heat map using FACS-isolated ZSGreen+ EVs from control versus mammary tumor-bearing mice (n = 3 mice/group). Asterisks indicate a few differentially-expressed miRNAs of interest. (b) Volcano plot of miRNAs detected in ZSGreen+ EC-EVs from control versus mammary tumor-bearing mice. Selected miRNAs of interest that were determined to be statistically significant are indicated at far right. (c) Cryo-EM images of EVs isolated from conditioned media of normal mammary gland (NECs) or C3-TAg mammary tumors (TECs). The scale bar applies to both panels. (d) qPCR analysis of selected miRNAs in the indicated EVs (n = 2 individual samples tested in triplicate) or in cellular fractions (n = 3). Results were analyzed using Student’s t-test, *p < 0.05.
