Figure 6
From: The Combined Strategy for iron uptake is not exclusive to domesticated rice (Oryza sativa)

Expression analysis of Fe deficiency-related genes in Sorghum bicolor. Relative expression levels (RT-qPCR, relative to SbACT expression) of selected genes ((A) IRT1; (B) NRAMP1; (C) IRO2; (D) YS1), in roots of plants submitted to control (CC) or Fe-deficiency (−Fe) conditions for three days. Roots were collected from sorghum plants grown in aerated nutrient solution, at three-leaf stage on both conditions at the time of RNA extraction. Values are the averages of four samples (3 plants each) ± SE. Asterisks indicate statistical difference between plants grown under CC and −Fe conditions (Student t-test, *P-value < 0.05, **P-value < 0.01).