Table 2 List of primers, annealing temperatures, and sequence sizes.
From: Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka
Gene | Primer sequence (5′-3′) | Annealing T (°C) | Amplicon size (bp) | Size (bp) after primers were removed | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
tams1 | ATGTTGTCCAGGACCACCCTC | TTAAAGGAAGTAAAGGACTGATGAGAAG | 56 | 843 | 794 |
tasp | ATGAAATTCTTCTACCTTTTTGTTCTATTTCC | TTAACAACAATCTTCGTTAATGCGAG | 65 | 1020 and 1023 | 962 and 965 |
18S rRNA (short) | AGGGCTAATACATGTTCGAGGCCATTT | TCCCTCTAAGAAGCGATAACGGGACAGT | 64 | 1185 | 1130 |
18S rRNA (long) | AAGCCATGCATGTCTAAGTATAAGCTTTT | GAATAATTCACCGGATCACTCG | 56 | 1587 | 1536 |
cob | ATGAATTTGTTTAACTCACATTTGC | TTATGCACGAACTCTTGCAGAG | 55 | 1092 | 1045 |
