Scientific Reports

Table 4 Effects of different treatments on the plasma cytokines of broiler chickens.

From: Effects of oxygen levels and a Lactobacillus plantarum strain on mortality and immune response of chickens at high altitude

Items¹	Day 7					Day 14
Items¹	IL-6, pg/ml	IL-1β, ng/ml	LITAF, ng/ml	IL-10, pg/ml	IL-12, pg/ml	IL-6, pg/ml	IL-1β, ng/ml	LITAF, ng/ml	IL-10, pg/ml	IL-12, pg/ml
Treatment
Low-oxygen
CON	106.32	4.43	2.57	450.68^b	102.29	343.05	5.78^a	3.52^a	493.86	195.61
AUR	109.20	4.42	2.39	472.36^b	90.19	310.04	4.55^ab	2.93^ab	532.62	185.08
PRO	116.04	4.35	1.84	554.66^a	90.27	302.55	4.30^b	2.59^b	588.50	184.29
High-oxygen
CON	104.95	2.85	1.80	487.23^b	91.15	308.94	4.36^a	2.82^a	605.37	188.96
AUR	103.80	2.90	1.34	602.50^ab	93.53	325.91	3.19^b	2.03^b	609.21	168.02
PRO	104.45	3.04	1.34	617.72^a	91.05	329.82	3.26^b	1.92^b	724.66	160.01
SEM²	1.51	0.20	0.07	13.25	3.03	9.95	0.15	0.11	16.84	5.26
Main effect oxygen (n = 18)
Low-oxygen	110.52	4.40^a	2.27^a	492.57^b	94.24	318.55	4.88^a	3.03^a	538.33^b	188.32
High-oxygen	104.40	2.93^b	1.49^b	569.15^a	91.91	321.56	3.60^b	2.25^b	646.41^a	172.34
Main effect Probiotic (n = 12)
CON	105.63	3.64	2.19^a	468.96^b	96.72	326.00	5.07^a	3.19^a	549.71^b	192.28
AUR	106.50	3.66	1.87^b	537.43^a	91.86	317.97	3.87^b	2.48^b	570.91^ab	176.55
PRO	110.24	3.70	1.59^b	586.19^a	90.66	316.19	3.78^b	2.25^b	656.58^a	172.17
P-values
Oxygen	0.050	0.001	0.019	0.008	0.700	0.881	0.001	0.003	0.005	0.150
Probiotic	0.416	0.992	0.012	0.006	0.713	0.921	0.007	0.011	0.035	0.316
Oxygen × Probiotic	0.388	0.948	0.386	0.313	0.619	0.460	0.864	0.902	0.757	0.807

¹LITAF: Lipopolysaccharide-induced TNF-α factor; High-oxygen: chickens were reared in a room with oxygen percentages between 25.8% and 26.5%; Low-oxygen: chickens were reared in a room with oxygen percentages between 20.7% and 21.4%. CON: chickens were fed basal diet; AUR: chickens were fed basal diet supplemented with aureomycin (50 mg/kg diet); PRO: chickens were fed basal diet and inoculated with L. plantarum (10⁹ CFU/chicken) by gavage on day 1. ²SEM: standard error of a mean (n = 6 pens per treatment group). ^a,bDifferent superscripts indicate significant differences (P < 0.05).

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