Figure 1 | Scientific Reports

Figure 1

From: Expansion of Human Pluripotent Stem Cell-derived Early Cardiovascular Progenitor Cells by a Cocktail of Signaling Factors

Figure 1

Characterization of CMCs generated from three hPSC lines. (a) Schematic diagram showing differentiation protocol used for CMC induction from hPSCs. hPSC lines were cultured in suspension as spheroids and differentiated into mesendoderm, followed by cardiac mesoderm lineages by one-day treatment with 12 µM CHIR99021 (CHIR) and a one-day rest period. (b) Morphology of the CMC spheroids derived from two hESC lines (RH5 and RH6) and an iPS line. Scale bars: 200 µm. (c) Percentages of MESP1+ cells in CMC spheroids derived from RH5, RH6, and iPS cells. Data: mean ± standard deviation (SD). (d) Expression analysis of cardiac mesodermal and cardiac-specific genes in CMC spheroids. Data presented as median.

Back to article page