Figure 2 | Scientific Reports

Figure 2

From: Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif

Figure 2

Biophysical characterization of the N-terminal part of JIP3. (a) Schematic representation of the different fragments of the N-terminal part of JIP3 used in this study. (b) Superposition of the MALS curves for RH1-LZI (black) and LZI (green) fragments of JIP3. (c,d) Characterization of the self-association of the JIP3-Nter dimers using fluorescence-detected sedimentation velocity (SV-FDS). Dilution series with fluorescent label for the (c) JIP3-RH1-LZI fragment and (d) JIP3-LZI fragment. Sedimentation coefficient distributions c(s) and for the LZI fragment the resulting sw isotherms (inset) from integration of c(s) are shown. In the isotherm plots, solid circles are the sw data from the dilution series, and the solid line is the best-fit isotherm.

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