Table 1 Quantification of average diffusion and binding probabilities for lipases and respective mutants.

From: Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition

Lipase

Total tracks

Average Diffusion

10−10 [cm²/s]*

Binding probability

Native

4.804

3.5 ± 1.7

31.6%

Lid mutation 3 (L3)

62.642

4.7 ± 1.1

32.1%

Lid mutation 2 (L2)

44.523

3.1 ± 1.0

51.2%

Native product

12.432

1.7 ± 1.1

31.2%

Lid mutation 3 (L3) product

3.013

2.8 ± 1.3

30.8%

DOPE-ATTO655 (SPT)

2.267

3.7 ± 0.01

na**

  1. *Error corresponds to one standard deviation.
  2. **Not extractable/ensemble.
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