Figure 1
From: Nanopore sequencing improves the draft genome of the human pathogenic amoeba Naegleria fowleri
Analysis of Benchmarking Universal Single-copy Orthologs (BUSCOs) at different assembly steps. The genome completeness was evaluated by analysing 303 conserved BUSCOs of the Eukaryota odb9 dataset. The initial Canu assembly contains high numbers of fragmented and missing BUSCOs. Polishing using Nanopolish in combination with ONT raw data and highly accurate Illlumina reads by applying Pilon could reduce the number of fragmented and missing BUSCOs. Further, manual curation using MUMmer alignments and duplicated BUSCOs reduced the occurrence of duplicated BUSCOs.
