Figure 3
From: Identifying inaccuracies in gene expression estimates from unstranded RNA-seq data
Comparison of DEGs called on strand-aware and strand-unaware counts. Three columns of Venn diagrams correspond to three comparisons of cell types indicated on top. Left circle (blue) always corresponds to “true” DEGs identified with strand-aware counts. Top row (A) shows overlap of DEGs obtained with strand-aware vs. strand-unaware counts. Middle row (B) is the same but only for strandedness-affected genes (those where log2(Nustno/Nstno) > 1). Bottom row (C) covers the same genes that are prone to expression overestimation used in B, but uses junction counts for differential expression analysis.
