Figure 2
From: Postmenopausal Osteoporosis reference genes for qPCR expression assays
Stability analysis as revealed using different software packages. (A) Candidate reference gene stability analyzed using GeNorm. Low M values predict high stability while high M values indicate low stability. (B) Pairwise variation (Vn/Vn + 1) to determine the optimal number of reference genes required for accurate normalization by GeNorm. In this OP study, the pairwise variation value less than the cut-off (0.15) is reached with two reference genes. (C) Candidate reference gene stability analyzed using NormFinder. Low M values predict higher stability. (D) Candidate reference gene stability analyzed using BestKeeper. High Pearson correlation coefficient (r) predicts high stability.
